RT-PCR is a technique as useful as it is ambiguously named. In this case, I’m referring to real-time PCR, also known as quantitative PCR (qPCR), but not to be confused with reverse transcriptase PCR ...
Are you new to quantitative (or “real-time”) PCR? Maybe you’re just looking to brush up on the basics of designing and analyzing qPCR experiments. Perhaps your reactions aren’t working. Again.
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