Real-time PCR (qPCR) analysis has become the method of choice for quantitating DNA in a wide variety of applications. The availability of commercial kits has made the technique easy to perform, ...

Active DNA demethylation in mammals involves oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). However, genome-wide detection ...

DNA size selection employs specific ratios to capture and separate fragments by size. Both single- and double-sided DNA size selections are integral to next generation sequencing (NGS) library ...

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

Site-directed mutagenesis (SDM) is a commonly used technique for introducing mutations into a gene of interest. Existing techniques for SDM, such as whole plasmid SDM, while effective, are time ...